3 μl purified phage lysate (1010 pfu/ml) was applied to a carbon-coated copper grid and negatively stained with 2% (w/v) uranyl acetate for 30 s and excess liquid was blotted off (Czajkowski et al., 2015 (link)). After air-drying the grids were visualized with FEI Tecnai 12 BioTwin Transmission Electron Microscope operating at 100 kV.
Free full text: Click here