Worms were anaesthetised using 2 μM levamisole and mounted on 5% agar pads. Images were collected using an Olympus BX53 or a Leica TCS SP8 X microscope. The GFP intensity of DAF-16::GFP::3xFLAG was measured by ImageJ (1.53t). Background signals were subtracted as reported77 (link).
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