The cellulose beads (100 g beads) were suspended in 400 or 200
mL of sodium periodate (NaIO4) at a concentration of 50
or 20 mM and reacted at 25 °C for 28 h under mild agitation using
an overhead stirrer to form DAC beads, and aliquots of beads were
withdrawn at 2, 4, 8, 24, and 28 h to calculate kinetics. Excess NaIO4 was removed by washing the beads (soaking and sieving with
a 1 mm mesh-size stainless-steel sieve) in abundant DI water
until the absorption of the supernatant at 290 nm was zero (periodate
adsorption peak). The dialdehyde beads (DAC beads) were stored in
DI water at room temperature. The DO was determined via acid–base
titration.20 (link) 2 g of oxidized beads were
homogenized with 3 mL of DI water using an Ika Ultra-Turrax T8 and
dispersed into 25 mL of a 0.25 M hydroxylamine hydrochloride solution
(adjusted with 0.1 M NaOH to pH 4). The HCl released in the reaction
between the aldehydes and hydroxylamine hydrochloride was titrated
against 0.1 M NaOH using an Accumet pH meter (Fisher Scientific),
and equivalent point peaks were obtained from the first order derivative
of pH changes against volume added (dpH/dV).
mL of sodium periodate (NaIO4) at a concentration of 50
or 20 mM and reacted at 25 °C for 28 h under mild agitation using
an overhead stirrer to form DAC beads, and aliquots of beads were
withdrawn at 2, 4, 8, 24, and 28 h to calculate kinetics. Excess NaIO4 was removed by washing the beads (soaking and sieving with
a 1 mm mesh-size stainless-steel sieve) in abundant DI water
until the absorption of the supernatant at 290 nm was zero (periodate
adsorption peak). The dialdehyde beads (DAC beads) were stored in
DI water at room temperature. The DO was determined via acid–base
titration.20 (link) 2 g of oxidized beads were
homogenized with 3 mL of DI water using an Ika Ultra-Turrax T8 and
dispersed into 25 mL of a 0.25 M hydroxylamine hydrochloride solution
(adjusted with 0.1 M NaOH to pH 4). The HCl released in the reaction
between the aldehydes and hydroxylamine hydrochloride was titrated
against 0.1 M NaOH using an Accumet pH meter (Fisher Scientific),
and equivalent point peaks were obtained from the first order derivative
of pH changes against volume added (dpH/dV).
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