Calibration mixtures were prepared
using lipid, small molecule, and polyalanine standards. The lipid
mixture was prepared in MeOH, 0.1% FA. The polyalanine mixture was
prepared in 1:1 MeOH/H2O, 0.1% FA. A combined lipid, small
molecule, and polyalanine mixture was also prepared in 1:1 MeOH/H2O, 0.1% FA. The individual lipid and polyalanine mixtures
along with the small molecules from the combined mixture were used
for the calibration seen in this manuscript. The combined mixture
as a whole was used for demonstration purposes. The final concentrations
for the standards can be found inSupporting Information Document 1, Table S2 . A 10× dilution of SPLASH Lipidomix
was prepared by adding 10 μL of the mixture, as obtained from
the vendor, to 90 μL of MeOH.
using lipid, small molecule, and polyalanine standards. The lipid
mixture was prepared in MeOH, 0.1% FA. The polyalanine mixture was
prepared in 1:1 MeOH/H2O, 0.1% FA. A combined lipid, small
molecule, and polyalanine mixture was also prepared in 1:1 MeOH/H2O, 0.1% FA. The individual lipid and polyalanine mixtures
along with the small molecules from the combined mixture were used
for the calibration seen in this manuscript. The combined mixture
as a whole was used for demonstration purposes. The final concentrations
for the standards can be found in
was prepared by adding 10 μL of the mixture, as obtained from
the vendor, to 90 μL of MeOH.
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