Bacterial DNA Extraction and Identification

The genomic DNA of the LAB isolates was extracted using a bacterial DNA extraction kit (Tiangen Biotech, Beijing, China) for molecular identification. The sequences of 16S rRNA were amplified using universal primers 27F and 1492R, as previously described [26 (link)]. The PCR products were sequenced by Sangon Biotech Co., Ltd. (Shanghai, China). The sequence data obtained were subjected to online homology analysis using NCBI BLASTn for the final identification of LAB.

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Li X., Li W., Zhao L., Li Y., He W., Ding K, & Cao P. (2024). Characterization and Assessment of Native Lactic Acid Bacteria from Broiler Intestines for Potential Probiotic Properties. Microorganisms, 12(4), 749.

Publication 2024

bacterial DNA extraction kit

Corresponding Organization :

Other organizations : Henan University of Science and Technology

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Variable analysis

independent variables

Bacterial DNA extraction kit (Tiangen Biotech, Beijing, China)

dependent variables

Sequences of 16S rRNA

control variables

Universal primers 27F and 1492R

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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