Modified Perl’s staining was performed on 14 μm sagittal sections of paraformaldehyde fixed brain as described in20 (link). Staining at different emission wavelength was evaluated by the Nuance® FX multiplex biomarker imaging system (PerkinElmer) in the choroid plexus of the mice. A semi-quantitative analysis of iron deposition normalized for cell staining was performed using ImageJ software (National Institute of Health, http://rsb.info.nih.gov/ij) as in ref. 20 (link).
Zanardi A., Nardini I., Raia S., Conti A., Ferrini B., D’Adamo P., Gilberti E., DePalma G., Belloli S., Monterisi C., Coliva A., Rainone P., Moresco R.M., Mori F., Zurlo G., Scali C., Natali L., Pancanti A., Giovacchini P., Magherini G., Tovani G., Salvini L., Cicaloni V., Tinti C., Tinti L., Lana D., Magni G., Giovannini M.G., Gringeri A., Caricasole A, & Alessio M. (2024). New orphan disease therapies from the proteome of industrial plasma processing waste- a treatment for aceruloplasminemia. Communications Biology, 7, 140.
Other organizations :
IRCCS Ospedale San Raffaele, Kedrion (Italy), University of Brescia, Toscana Life Sciences, University of Florence, Nello Carrara Institute of Applied Physics, National Research Council
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