Synergistic Effects of Kinase Inhibitors

Cells were grown in medium supplemented with 10% FBS and relative number of viable cells was measured using WST1 reagent from Millipore Sigma. WST1 was added 2 h prior to absorbance measurement at 450 nm using a Tecan Infinite M200 PRO microplate reader and TECAN i-control software. Cells were plated and after adhesion were treated with THZ531 (5–1000 nM or 100 nM) or THZ1 (0.5–60 nM) in addition to BI601245, MLN120B, AS601245 or JNK-IN-8 at the indicated concentration. Cells were grown for 72 h before addition of WST1. The results displayed represent the mean of n = 5 or 6 ± standard deviation (SD). Bliss scores were calculated using SynergyFinder 2.0^{78 (link)}.