Genotyping of DRD2/ANKK1 and COMT Polymorphisms

Genomic DNA was extracted from blood leukocytes using the KingFisher^™ Duo Prime Purification System (Thermo Scientific^™) according to the manufacturer’s protocol. Genotyping of the SNPs DRD2/ANKK1 TaqIA (NCBI accession number: rs1800497) and COMT Val108/158Met (rs4680) was performed using PCR-based restriction fragment length analysis according to previously described protocols (Schott et al. 2006 (link); Wimber et al. 2011 (link); Richter et al. 2013 (link), 2014 (link), 2017 (link)). A1 carriers of the TaqIA SNP were grouped together (A1 + : A1/A1 and A1/A2; A1 − : A2/A2) as in the previous studies (Klein et al. 2007 (link); Frank and Hutchison, 2009 (link); Jocham et al. 2009 (link); Stelzel et al. 2010 (link); Stice et al. 2012 (link); Richter et al. 2013 (link), 2014 (link), 2017 (link)).

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Richter A., de Boer L., Guitart-Masip M., Behnisch G., Seidenbecher C.I, & Schott B.H. (2021). Motivational learning biases are differentially modulated by genetic determinants of striatal and prefrontal dopamine function. Journal of Neural Transmission, 128(11), 1705-1720.

Publication 2021

KingFisher™ Duo Prime Purification System

Blood leukocytes Comt Drd2 Genomic

Corresponding Organization : Leibniz Institute for Neurobiology

Other organizations : Max Planck Institute for Human Development, Karolinska Institutet, Prostate Cancer Research, University College London, Center for Behavioral Brain Sciences, German Center for Neurodegenerative Diseases, University Hospital Magdeburg

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Variable analysis

independent variables

DRD2/ANKK1 TaqIA SNP (rs1800497)
COMT Val108/158Met SNP (rs4680)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

A1 carriers of the TaqIA SNP were grouped together (A1 + : A1/A1 and A1/A2; A1 − : A2/A2) as in the previous studies

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