SARS-CoV-2 testing was performed on nasopharyngeal swab samples. Upper respiratory samples were transported in a universal transport medium for viruses. Nucleic acid extraction was performed using the NucliSens easyMAG system (bioMérieux, Boxtel, The Netherlands). A real-time reverse transcription-polymerase chain reaction was processed on Applied Biosystems 7500 thermocycler (Foster City, CA, USA) using primers and conditions described elsewhere [13 (link)].
Martinez-Guerra B.A., Gonzalez-Lara M.F., de-Leon-Cividanes N.A., Tamez-Torres K.M., Roman-Montes C.M., Rajme-Lopez S., Villalobos-Zapata G.I., Lopez-Garcia N.I., Martínez-Gamboa A., Sifuentes-Osornio J., Ortiz-Brizuela E., Ochoa-Hein E., Galindo-Fraga A., Bobadilla-del-Valle M, & Ponce-de-León A. (2021). Antimicrobial Resistance Patterns and Antibiotic Use during Hospital Conversion in the COVID-19 Pandemic. Antibiotics, 10(2), 182.
Presence or absence of SARS-CoV-2 in nasopharyngeal swab samples
control variables
Nasopharyngeal swab sample collection
Transport of samples in universal transport medium for viruses
Nucleic acid extraction using the NucliSens easyMAG system
Real-time reverse transcription-polymerase chain reaction on Applied Biosystems 7500 thermocycler
Primers and conditions described elsewhere [13]
positive controls
Not explicitly mentioned
negative controls
Not explicitly mentioned
Annotations
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