Lipid Analysis in Caco-2 Cells

Total lipids in Caco-2 cells, AC and BLC were extracted, and relative fatty acids methylated as previously described [18 (link)]. Before methylation, pentadecanoic acid was added as internal standard. The qualitative and quantitative content of fatty acid methyl esters (FAMEs) was determined by fast-GC (GC-2030AF; Shimadzu, Kyoto, Japan) using a capillary column (30 mt, 0.2 μm film thickness) with a programed temperature gradient (50–250 °C, 10 °C/min). The gas chromatographic peaks were identified based on their retention time ratios relative to methyl stearate and predetermined by use of authentic samples [19 (link)]. Gas chromatographic traces and quantitative evaluations were obtained using a Lab Solution software (Shimadzu, Kyoto, Japan).