Protocol detail

EHEC O157:H7 Detection Protocol

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A reference strain of EHEC O157:H7 (ATCC 700728) was obtained from Jordan Food and Drug Administration (Amman, Jordan) and was used as a positive control in the study. Bacterial culture was performed according to previously published protocols [3 (link),12 ]. Briefly, fecal swabs were inoculated into 9 mL of Mueller-Hinton broth (Oxoid, UK) and incubated at 37°C for 24 h. Then, a loopful was streaked on MacConkey agar medium (Oxoid) and on eosin methylene blue (Oxoid) and incubated overnight at 37°C. Colonies of E. coli were identified by biochemical reactions using IMViC test (Tulip Diagnostics, India).

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Tarazi Y.H., El-Sukhon S.N., Ismail Z.B, & Almestarehieh A.A. (2021). Molecular characterization of enterohemorrhagic Escherichia coli isolated from diarrhea samples from human, livestock, and ground beef in North Jordan. Veterinary World, 14(10), 2827-2832.

Publication 2021

Mueller-Hinton broth MacConkey agar medium eosin methylene blue

Agar Bacterial Diagnostics E coli Ehec Eosin Fecal Methylene blue Strain Tulip

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Variable analysis

independent variables

Bacterial culture protocol

dependent variables

Identification of E. coli colonies by biochemical reactions using IMViC test

control variables

Reference strain of EHEC O157:H7 (ATCC 700728) obtained from Jordan Food and Drug Administration (Amman, Jordan)
Fecal swabs inoculated into Mueller-Hinton broth and incubated at 37°C for 24 h
Loopful streaked on MacConkey agar medium and eosin methylene blue and incubated overnight at 37°C

positive control

Reference strain of EHEC O157:H7 (ATCC 700728)

negative control

Not explicitly mentioned

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