Protocol detail

CD Spectroscopy of CP12 Protein

Find Similar Protocols

10 µM CP12 without histidine tag in 50 mM sodium phosphate pH 6.5 was used to record CD spectra (Jasco 815CD spectrometer, 2 mm thick quartz cells) scanned from 190 to 260 nm at a speed of 10 nm/min (n = 3). The data were analyzed using Dichroweb using the CDSSTR, SELCON3 and CONTIN analysis programs, and the reference set 7 (http://dichroweb.cryst.bbk.ac.uk/html/home.shtml) [40 (link), 41 (link)] and Bestsel (http://bestsel.elte.hu/index.php) [42 (link)].

Free full text: Click here

Shao H., Huang W., Avilan L., Receveur-Bréchot V., Puppo C., Puppo R., Lebrun R., Gontero B, & Launay H. (2021). A new type of flexible CP12 protein in the marine diatom Thalassiosira pseudonana. Cell Communication and Signaling : CCS, 19, 38.

Publication 2021

815CD spectrometer

Cells Histidine Quartz Sodium phosphate

Top 5 similar protocols

Variable analysis

independent variables

Concentration of CP12 (10 µM)

dependent variables

CD spectra scanned from 190 to 260 nm

control variables

Sodium phosphate buffer (50 mM, pH 6.5)
Quartz cells with 2 mm thickness
Scanning speed of 10 nm/min
Number of replicates (n = 3)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!