Quantitative Analysis of Cardiac Genes

The total RNA in heart tissue was extracted by the animal RNA Isolation Kit and then the RNA concentration was determined. The reverse transcription kit was used to reverse transcribe the RNA into cDNA. The cDNA was used as a template and then the target genes were amplified by PCR. Glyceraldehyde phosphate dehydrogenase (GAPDH) was used as an internal reference primer for the target genes. The expression of target genes was analyzed by the 2^−ΔΔCT method [23 (link)]. The Primer sequences: GAPDH forward primer (5′-AATGGATTTGGACGATTGGT-3′) and reverse primer (5′-TTTGCACTGGTACGTGTTGAT-3′), brain natriuretic peptide (BNP) forward primer (5′-GAGGTCACTCCTATCCTCTGG-3′) and reverse primer (5′-GCCATTTCCTCCGACTTTTCTC-3′), atrial natriuretic peptide (ANP) forward primer (5′-GCTTCCAGGCCATATTGGAG-3′), and reverse primer (5′-GGGGGCATGACCTCATCTT-3′).