Immunohistochemical Profiling of Tissue Samples

Hematoxylin–eosin staining reagent was purchased from Beijing Zhongshan Jinqiao Co., Ltd. (Beijing, China). Antibodies were purchased from Fuzhou Maixin Biotechnology Development Co., Ltd. (Fuzhou, China) (Table 1). Immunohistochemical staining was performed by EnVision's two-step method. The main immunohistochemical markers were CKpan, CD34, CD31, FLI-1, EMA, INI-1, S100, SOX-10, HHV8, Desmin and Ki-67. Two pathologists re-evaluated the sections.Table 1

Antibodies used in immunohistochemistry and their sources

Antibody	Clone number	Monoclonal/Polyclonal	Positive site
CK (pan)	MX005	monoclonal	cytoplasm
CD34	QBEnd/10	monoclonal	cell membrane and cytoplasm
CD31	JC/70A	monoclonal	cell membrane
FLI-1	MX045	monoclonal	nucleus
ERG	MXR004	monoclonal	nucleus
INI-1	MRQ-27	monoclonal	nucleus
S100	4C4.9	monoclonal	cytoplasm/nucleus
SOX-10	EP268	monoclonal	nucleus
Desmin	D33	monoclonal	cytoplasm
Vimentin	V9	monoclonal	cytoplasm
EMA	E29	monoclonal	cytoplasm/cell membrane
HHV8	13B10	monoclonal	nucleus

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Yang N., Huang Y., Yang P., Yan W., Zhang S., Li N, & Feng Z. (2023). Clinicopathological study of pseudomyogenic hemangioendothelioma. Diagnostic Pathology, 18, 25.

Publication 2023

Antibodies Cell Desmin Eosin Hhv8 Immunohistochemistry Membrane Pathologists S100

Corresponding Organization :

Other organizations : Second Hospital of Anhui Medical University, Anhui Medical University, Lu'an First People's Hospital, Third People's Hospital of Hefei

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Variable analysis

independent variables

Hematoxylin–eosin staining reagent
Antibodies

dependent variables

Immunohistochemical staining results
Evaluation of sections by two pathologists

control variables

EnVision's two-step method for immunohistochemical staining

controls

Positive controls: Not specified
Negative controls: Not specified

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