Cell fixation and immuofluoresence (IF) staining were performed as described previously (6 (link),39 (link),40 (link)). Cells were fixed during vegetative growth or at 10 h after mixing cells of two mating types, as indicated. For the 6mA staining, fixed cells were treated with RNase A (50 μg/ml or otherwise indicated) in phosphate-buffered saline (PBS) buffer for 2 h at 37°C. Non-treated cells were either incubated at 37°C or at room temperature (RT), as indicated. Cells were then incubated with 2N HCl for 20 min at RT and neutralized with Tris–HCl (100 mM, pH 8.5) for 10 min, prior to antibody incubation. The primary antibodies are α-6mA (Synaptic Systems, 202003, 1:2000) (5 (link),6 (link),10 (link)), α-HA (Cell Signaling, C29F4, 1:200), α-H3K9-acetylation (Abcam, ab10812, 1:500) and α-H3K14-acetylation (Abcam, ab52946, 1:500). The primary antibodies are incubated with cells at 4°C overnight (for α-6mA) or at RT for 2 h (for other antibodies). Cells were then incubated in the secondary antibody (Goat anti-Rabbit IgG (H+L), Invitrogen, A-21428, 1:4000) at RT for 1 h. Digital images were collected using a Leica DM2500 microscope with a Leica DFC450C camera.