Biotelemetry for Circadian Rhythms

Eight-week-old male C57BL/6 mice were used for measurement of body temperature and locomotor activity using biotelemetry transmitters (E-mitter, STARR Life Science Corp., Oakmont, PA, USA) as previously described [34 (link)]. Briefly, mice were anesthetized with tribromoethanol (250 mg/kg, Sigma-Aldrich) and a biotelemetry transmitter was implanted into the abdominal cavity. Output was monitored by a receiver (ER-4000, STARR Life Science Corp.) laid under each cage. A data acquisition system (Vital View, STARR Life Science Corp.) was used for automatic data collection and analysis. Mice were adapted to the vital view system for 24 h prior to SPX injection.