Characterization of Mesenchymal Stem Cells

Flow cytometry was performed by using an LSRII four-laser flow cytometer (BD Biosciences, Oxford, UK) with appropriate isotype controls. Passaged MSCs (p3 from HN, SF, and ICBM) (n = 3 for each group) were stained with combinations of the following antibodies at the dilution recommended by the manufacturers: anti-CD14-allophycocyanin-cyanine (APC-H7), anti-CD19-fluorescein isothiocyanate (FITC), anti-CD34-peridinin chlorophyll protein (PerCP), anti-HLADR-phycoerythrin-cyanine (PE-Cy7), anti-CD73-phycoerythrin (PE), anti-CD90-PE, anti-CD81-FITC, anti-CD44-FITC, and anti-CD29-FITC (all from BD Biosciences). Cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) as a live/dead discriminator immediately prior to acquisition
[15 (link)]. At least 10,000 live cell events were collected for each antibody combination.

Free full text: Click here

Baboolal T.G., Boxall S.A., Churchman S.M., Buckley C.T., Jones E, & McGonagle D. (2014). Intrinsic multipotential mesenchymal stromal cell activity in gelatinous Heberden’s nodes in osteoarthritis at clinical presentation. Arthritis Research & Therapy, 16(3), R119.

Publication 2014

LSRII four-laser flow cytometer anti-CD90-PE anti-CD44-FITC anti-CD29-FITC 4′,6-diamidino-2-phenylindole (DAPI)

Allophycocyanin Antibodies Antibody Cd44 Cd73 Cd90 Cell Chlorophyll Dilution Flow cytometry Fluorescein Isothiocyanate Isotype Peridinin Phycoerythrin Protein

Corresponding Organization :

Other organizations : Chapel Allerton Hospital, University of Leeds, Trinity College Dublin, NIHR Leeds Musculoskeletal Biomedical Research Unit

Top 5 similar protocols

Variable analysis

independent variables

Cell source (HN, SF, and ICBM)

dependent variables

Expression of surface markers (CD14, CD19, CD34, HLA-DR, CD73, CD90, CD81, CD44, CD29)

control variables

Passage number (p3)
Number of samples per group (n = 3)
Flow cytometry analysis (LSRII four-laser flow cytometer, BD Biosciences)
Staining with isotype controls
Live/dead discrimination with DAPI staining
Minimum of 10,000 live cell events collected per antibody combination

controls

Isotype controls
DAPI (live/dead discriminator)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!