Immunohistochemistry of Embryonic Membranes

Freshly isolated E18/E19 membranes were fixed and embedded as described previously.^{15 (link)} Membrane cryosections of 10-µM thickness were stained for the expression of proliferin, periostin, and α-fetoprotein (AFP) antibodies (Santa Cruz Biotechnology). Nuclei were counterstained with ProLong Gold antifade reagent with 6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific). Images were captured on Leica Microsystems CTR6500 and further analyzed using NIH-ImageJ software.

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Togarrati P.P., Dinglasan N., Yee E., Heitman J.W., Jackman R.P., Geisberg M., Norris P.J., Bárcena A, & Muench M.O. (2019). Potential of Membranes Surrounding the Fetus as Immunoprotective Cell-Carriers for Allogeneic Transplantations. Transplantation Direct, 5(6), e460.

Publication 2019

CTR6500

Antibodies Cryosections Gold Membrane Nuclei Periostin

Corresponding Organization : University of California, San Francisco

Other organizations : Silver Lake Research (United States), Reproductive Science Center

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Variable analysis

independent variables

Membrane cryosections

dependent variables

Expression of proliferin
Expression of periostin
Expression of α-fetoprotein (AFP)

control variables

Thickness of membrane cryosections (10-µM)
Freshly isolated E18/E19 membranes
Staining with antibodies (Santa Cruz Biotechnology)
Nuclei counterstained with ProLong Gold antifade reagent with 6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific)
Imaging with Leica Microsystems CTR6500
Image analysis using NIH-ImageJ software

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