In addition to the NRG2 antibodies described above, the commercial and custom antibodies against the following proteins were used in this study: ErbB4, mouse monoclonal Ab-77 (Thermo Fisher; 1 μg/ml), rabbit monoclonal mAB10 (1 μg/ml) 7 (link) and rabbit polyclonal custom antibody 5721 (unpublished; 0.4 μg/ml); Kv2.1, mouse monoclonal (clone K89/34; NeuroMab; 1 μg/ml); rabbit polyclonal antibody against NRG1 (SC-348; Santa Cruz Biotechnology; 0.2 μg/ml); anti-V5 epitope tag (AbD Serotech; 1 μg/ml); Bassoon, rabbit polyclonal (Synaptic Systems; 1:1,000); Gephyrin, mouse monoclonal (clone mAb7a; Synaptic Systems; 1:500); PSD95, mouse monoclonal (clone 7E3-1B8; Pierce; 1:500); Calbindin, mouse monoclonal (clone CB-955; Sigma; 1:1,000); GFP, rabbit polyclonal (Molecular Probes; 1:2,000); Erk2, rabbit polyclonal (C-14; Santa Cruz; 0.2 μg/ml); phospho-Erk2, mouse monoclonal (clone E-4; Santa Cruz; 0.2 ug/ml); GAPDH, mouse monoclonal (clone 6C5; Santa Cruz; 0.2 μg/ml); clathrin heavy chain (CHC), mouse monoclonal (SC-12734; Santa Cruz; 0.1 μg/ml); GluN2A, rabbit monoclonal (clone A12W; Millipore; 1:2,000); GluN2B (clone 13; BD Biosciences; 1 μg/ml); GluA1 (AB1504; Millipore; 0.2 μg/ml).
Protocol detail
Antibody Characterization for Neuroscience Research
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Antibodies
Antibody
Calbindin
Clathrin heavy chain
Clone
Epitope
Erk2
Gapdh
Gephyrin
Glun2a
Glun2b
Molecular probes
Mouse
Nrg1
Nrg2
Proteins
Rabbit
Corresponding Organization :
Other organizations : Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institute of Neurological Disorders and Stroke
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Variable analysis
independent variables
- ErbB4 antibodies: mouse monoclonal Ab-77 (1 μg/ml), rabbit monoclonal mAB10 (1 μg/ml), and rabbit polyclonal custom antibody 5721 (0.4 μg/ml)
- Kv2.1 antibody: mouse monoclonal (clone K89/34; 1 μg/ml)
- NRG1 antibody: rabbit polyclonal (0.2 μg/ml)
- V5 epitope tag antibody: (1 μg/ml)
- Protein expression or localization of the following targets: ErbB4, Kv2.1, NRG1, and V5 epitope tag
- Bassoon (1:1,000)
- Gephyrin (1:500)
- PSD95 (1:500)
- Calbindin (1:1,000)
- GFP (1:2,000)
- Erk2 (0.2 μg/ml)
- Phospho-Erk2 (0.2 μg/ml)
- GAPDH (0.2 μg/ml)
- Clathrin heavy chain (0.1 μg/ml)
- GluN2A (1:2,000)
- GluN2B (1 μg/ml)
- GluA1 (0.2 μg/ml)
- NRG2 antibodies described in the study
- Not explicitly mentioned
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