At 14, 70, and 90 dpi, the hearts of uninfected and MCMV-infected mice were evaluated by echocardiography (Toshiba Aplio 80, Tokyo, Japan). Briefly, mice were sedated with 2% isoflurane via nose cone inhalation and the heart rate, left ventricular internal diameter (diastolic/systolic), and left ventricular posterior wall thickness (diastolic/systolic) were measured [15 (link),41 (link)].
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