We prospectively collected clinical and pathological data from consecutive consenting patients undergoing radical prostatectomy for prostate cancer between 2014 and 2015. Surgical specimens underwent conventional histological processing and examination according to the Stanford protocol. Additionally, in each tissue section, areas of adenocarcinoma were identified and graded according to Gleason Score. Then, the Index lesion corresponding to the highest Gleason Score was studied by immunohistochemistry, using anti-SIRT7 antibody (Prestige Antibodies®, SIGMA-ALDRICH®). SIRT7 expression was analyzed in a qualitative manner by evaluating positivity or negativity of expression, comparing healthy and tumor areas, and in a quantitative manner. To quantify the expression of SIRT7 we used the Allred Score (0 to 8) [10 (link)] obtained by adding proportion of staining tumor (0 to 5) and signal intensity (0 to 3). The quantification was done by determining the average intensity and proportion of nuclei labelled including the nucleolus.
In each index lesion, the Allred Score and Gleason Score were compared using the Student t test (significance with p < 0.05). The analysis was performed blindly by two independent investigators.
In each index lesion, the Allred Score and Gleason Score were compared using the Student t test (significance with p < 0.05). The analysis was performed blindly by two independent investigators.
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