Genomic DNA extracted from strain 10Alg 79T with the NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany) was used to amplify 16S rRNA genes with 27F (5′-AGAGTTTGATCMTGGCTCAG-3′) and 1492R (5′-TACGGTTACCTTGTTACGACTT-3′) primers [25 ] and sequences using an ABI Prism 3130xL DNA analyzer (Applied Biosystems, Hitachi, Japan).
The analysis of 16S rRNA gene sequences was performed on the EzBioCloud server [26 (link)], and the phylogenetic analysis was conducted using MEGA X software, version 10.2.1 [27 (link)], with the neighbor-joining (NJ), maximum-likelihood (ML), and maximum-parsimony (MP) methods. Genetic distances were calculated according to the Kimura two-parameter model [28 (link)], and bootstrap values were obtained from 500–1000 alternative trees.
The maximum-likelihood phylogeny of RpoC sequences translated from genome sequences was calculated using the IQ-TREE web server, version 1.6.12 [29 (link)], with 100 non-parametric bootstrap replicates and the LG+I+G4 substitution model determined using ModelFinder [30 (link)].
The analysis of 16S rRNA gene sequences was performed on the EzBioCloud server [26 (link)], and the phylogenetic analysis was conducted using MEGA X software, version 10.2.1 [27 (link)], with the neighbor-joining (NJ), maximum-likelihood (ML), and maximum-parsimony (MP) methods. Genetic distances were calculated according to the Kimura two-parameter model [28 (link)], and bootstrap values were obtained from 500–1000 alternative trees.
The maximum-likelihood phylogeny of RpoC sequences translated from genome sequences was calculated using the IQ-TREE web server, version 1.6.12 [29 (link)], with 100 non-parametric bootstrap replicates and the LG+I+G4 substitution model determined using ModelFinder [30 (link)].
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