Multi-Omics Analysis of Cerebellar and DLPFC Tissues

Genomic DNA was extracted from 100 mg of pulverized cerebellar tissue and DLPFC tissue with the phenol-chloroform method. SNP genotyping with HumanHap650Y, Human 1M-Duo BeadChips, HumanOmni5-Quad or HumanOmni2.5–8 (Illumina, San Diego, CA) was carried out according to the manufacturer’s instructions with DNA extracted from cerebellar tissue. The methylation and genotype data were extracted from a larger data set from one of our recent studies^{52 (link)}. In brief, methylation of DNA extracted from DLPFC was assessed according to the manufacturer’s instruction using the Infinium HumanMethylation450 BeadChip Kit (Illumina), which measure DNA methylation of more than 485,000 CpG dinucleotides (cg) covering 99% RefSeq gene promoters, including the CNR1 gene. The methylation data were processed and normalized using the minfi Bioconductor package in R^{53 (link)} as previously described^{52 (link)}.