Quantitative Western Blot Analysis

Western blot analysis was performed as previously described^{16 (link)}. Protein expression of LIPG was examined by western blotting using a mouse antibody against LIPG (Abcam, ab56493). Protein expression was detected by chemiluminescence (ECL, Pierce). Expression of α-tubulin (Thermo Fisher Scientific) was used as a protein loading control. Western blot data were quantified by densitometric analysis of autoradiograms, using a computerized densitometer (Typhoon System; Molecular Dynamics, Inc., Sunnyvale, CA). The quantitative protein level data were normalized by the α-tubulin protein levels.