Propagation of Herpesviruses and Poliovirus in Vero Cells

Vero cells (ATCC CCL-81, Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Microtech, Naples, Italy) supplemented with antibiotic solution 100× (100 IU/mL penicillin and 100 μg/mL streptomycin; Himedia, Naples, Italy), and 10% Fetal Bovine Serum (FBS, Microtech). HSV-1 (strain SC16), containing a lacZ gene driven by the cytomegalovirus IE-1 promoter to express β-galactosidase, HSV-2 (strain G, ATCC VR-734), and fluorescent HSV-1, containing the GFP reporter inserted into the gene coding for the VP22 tegument protein [30 (link)], were propagated on Vero cells, as previously reported [31 (link)]. As an unenveloped virus, Enterovirus C (Sb-1, poliovirus Sabin strain chat, ATCC VR-1562) was cultured on the Vero cell line.