DOCK11 Knockdown in HepAD38 Cells

DOCK11-target shRNA and a non-target shRNA control (Sigma-Aldrich, St. Louis, MO, USA) were used for the knockdown of the DOCK11 gene in HepAD38 cells, as previously described, but with slight modifications [10 (link)]. Briefly, shRNA was cloned into a lentivirus-based pLKO.1-puro shRNA expression vector. On Day 0, the lentiviral particle supernatant was added to the cells, and they were incubated overnight at 37 °C. On Day 1, the cells were washed twice with PBS, and medium for puromycin selection was added. From Days 3–6, the culture medium was replaced as appropriate. On Day 6, the cells were collected for RNA and DNA isolation or passaged to an 8-well chamber for hybridization.