Campanula Flower Scent Profiling

In order to characterize the flower scent chemistry of Campanula taxa, the headspace samples were analyzed on a Varian Saturn 2000 mass spectrometer coupled to a Varian 3800 gas chromatograph (GC/MS) equipped with a 1079 injector (Varian Inc., Palo Alto, CA, USA), which had been fitted with the ChromatoProbe kit [see 91] and a fused silica column ZB-5 (5% phenyl polysiloxane; 60 m long, inner diameter 0.25 mm, film thickness 0.25 µm, Phenomenex). Each quartz microvial was loaded into the probe, which was then inserted into the modified GC injector. Equipment configurations were identical to those published elsewhere [40 (link)].
Identification of compounds was carried out by using the NIST 08, Wiley 7 or Adams [92 ] mass spectral databases or the database provided in MassFinder 3 and were confirmed by a comparison of retention times with published data [92 ]. The structure assignments of individual components were confirmed by a comparison of mass spectra and GC retention times with those of available standards.
The composition of volatiles collected from flowering plants was compared with that from non-flowering plants (vegetative parts). Floral scent volatiles were those detected either at higher amounts (i.e., peak areas in chromatrogram of floral scent samples at least 10-fold larger than in leave samples) or exclusively in floral scent samples.