Genetic Profiling from Fasted Blood

Blood samples were collected from the antecubital vein after the subjects had fasted for ≥8 h. Part of the collected samples was used to examine biochemical indicators such as serum lipid levels, whereas the other part was transferred into a test tube containing anticoagulant solution to extract DNA. DNA was extracted using Tiangen Blood Genomic DNA extraction kits (Tiangen Biotech, Beijing, China), we use a PCR-LDR method to genotyping analysis as we described in detail in our previous study [27 (link)].

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Li Z., Ye C.Y., Wang L., Li J.M, & Yang L. (2020). Association of Genetic and Environmental Factors with Non-Alcoholic Fatty Liver Disease in a Chinese Han Population. International Journal of Environmental Research and Public Health, 17(14), 5217.

Publication 2020

Tiangen Blood Genomic DNA extraction kits

Anticoagulant Blood Collected samples Genomic Genotyping method Lipid Serum Vein

Corresponding Organization : Hangzhou Normal University

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Variable analysis

independent variables

Fasting duration (≥8 h)

dependent variables

Serum lipid levels
DNA genotyping

control variables

Antecubital vein for blood sample collection

controls

Positive control: Not specified
Negative control: Not specified

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