HE staining was conducted according to the routine protocol [17 (link)]. In brief, sections were stained with hematoxylin solution (Servicebio, China) for 5 min followed by five dips in 1% acid ethanol (1% HCl in 70% ethanol), and then rinsed in distilled water. Subsequently, the sections were stained with eosin solution for 3 min followed by dehydration with graded alcohol and clearing in xylene. The stained slices were photographed and examined using an optical microscope (Olympus, Japan).
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