Characterization of Dental Pulp Stem Cells

Human dental pulp stem cells (DPSC) were obtained from Lonza (PT-5025). The stemness of human dental pulp stem cells (DPSCs) (p. 16) was confirmed by flow cytometry as previously described [30 (link)]. The expression of positive (CD105) and negative (CD33) markers of mesenchymal stem cells was analyzed, using a BD FACS Canto II flow cytometer (BD Biosciences, San Jose, CA, USA). Briefly, cells were stained with PE anti-human CD105 antibody (323205, Biolegend, San Diego, CA, USA) and APC anti-human CD33 antibody (303407, Biolegend, San Diego, CA, USA), following the manufacturer’s instructions.

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Nguyen H.Q., Kao C.Y., Chiang C.P., Hung Y.H, & Lo C.M. (2022). Investigating the Immunomodulatory Potential of Dental Pulp Stem Cell Cultured on Decellularized Bladder Hydrogel towards Macrophage Response In Vitro. Gels, 8(3), 187.

Publication 2022

BD FACS Canto II flow cytometer PE anti-human CD105 antibody APC anti-human CD33 antibody

Anti antibody Cells Dental pulp Flow cytometry Human Mesenchymal stem cells Stem cells

Corresponding Organization : National Defense Medical Center

Other organizations : Tri-Service General Hospital, National Yang Ming Chiao Tung University

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Expression of positive (CD105) and negative (CD33) markers of mesenchymal stem cells

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