The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay was performed as described previously (29 (link)). Briefly, 48 h after transfection with negative control or ZNF132-specific siRNAs, cells were treated with CellTiter 96 Aqueous One Solution Reagent (Promega, Madison, WI). After 1 h of treatment, the optical density was measured at 490 nm on a GloMax®-Multi+ Detection System Glomax (Promega). Results were presented as the mean ± standard deviation for three separate determinations.
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MTS Assay for Cell Viability
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Corresponding Organization : Keio University
Other organizations : Kansai Medical University, Tokyo National Hospital
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Variable analysis
independent variables
- Negative control or ZNF132-specific siRNAs
- Cell viability
- Cells were treated with CellTiter 96 Aqueous One Solution Reagent (Promega, Madison, WI) for 1 h
- Negative control siRNAs
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