We extracted RNA from the NPCs and brain organoids at 2, 4, 8, and 10 weeks of differentiation using the Quick RNA extraction kit (Zymo). We quantified the integrity of RNA with NanoDrop. We reverse-transcribed cDNA using M-MLV Reverse Transcriptase (Promega) and random hexamer primers as described previously (44 (link)). We used the TaqMan gene expression assay to perform real-time polymerase chain reaction (RT-PCR).
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