Where indicated, BMDMs were transduced with either a control vector, wild-type human MMP9, a catalytically inactive MMP9 E402/A mutant (Orgaz et al., 2014 (link)), wild-type human MMP14, a catalytically inactive MMP14 E240/A mutant expression vector (Shimizu-Hirota et al., 2012 (link)), or a constitutively active human RhoA L63 expression vector (RTV-204; Cell Biolabs). The MMP9 and MMP14 constructs were cloned into the pLentilox-IRES-GFP lentiviral vector, while the RhoA constructs were inserted into pBABEhygro retroviral vector. Expression of the recombinant proteins was confirmed by Western blot.
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Manipulation of MMP9, MMP14, and RhoA in BMDMs
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Corresponding Organization : Wuhan University
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Variable analysis
independent variables
- Control vector
- Wild-type human MMP9
- Catalytically inactive MMP9 E402/A mutant
- Wild-type human MMP14
- Catalytically inactive MMP14 E240/A mutant
- Constitutively active human RhoA L63 expression vector
- Not explicitly mentioned
- BMDMs (bone marrow-derived macrophages)
- Positive control: Wild-type human MMP9, wild-type human MMP14, constitutively active human RhoA L63 expression vector
- Negative control: Control vector
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