Analytical Characterization of Folate Analogs

Tritiated pralatrexate, pemetrexed (both generally labeled), and [3′,5′,7-³H]MTX were obtained from Moravek Biochemicals (Brea, CA). Pemetrexed and MTX purity was established and monitored by high-performance liquid chromatography (HPLC) as described previously [24 (link)]. Pralatrexate purity was monitored using a 5 mm OSD2 4.6 × 250 mm reversed phase high-performance liquid chromatography column (Waters Spherisorb), by isocratic elution with 100 mM sodium acetate pH 5.5 (solvent A) and 15% acetonitrile (solvent B). The mobile phase was delivered at 1 ml/min, reaching 100% solvent B in 30 min. Nonlabeled MTX was obtained from Sigma-Aldrich (St. Louis, MO), nonlabeled (6S)5-formyltetrahydrofolate (5-formylTHF) from Schircks Laboratories (Jona, Switzerland) and nonlabeled pemetrexed from LC Laboratories (Woburn, MA). Pralatrexate was obtained from Spectrum Pharmaceuticals (Irvine, CA).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Visentin M., Unal E.S., Zhao R, & Goldman I.D. (2013). The membrane transport and polyglutamation of pralatrexate, a new-generation dihydrofolate reductase inhibitor. Cancer chemotherapy and pharmacology, 72(3), 597-606.

Publication 2013

5 formyltetrahydrofolate Acetonitrile High performance liquid chromatography Pharmaceuticals Pralatrexate Reversed phase liquid chromatography Sodium acetate Solvent

Corresponding Organization :

Other organizations : Albert Einstein College of Medicine, Jacobi Medical Center

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Tritiated pralatrexate
Pemetrexed (generally labeled)
[3',5',7-3H]MTX

dependent variables

Purity of pemetrexed
Purity of MTX
Purity of pralatrexate

control variables

Nonlabeled MTX
Nonlabeled (6S)5-formyltetrahydrofolate (5-formylTHF)
Nonlabeled pemetrexed

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!