Ketamine hydrochloride was purchased from Tocris/Bio-Techne (Warsaw, Poland) and psilocybin was synthesized at the Department of Medicinal Chemistry of the Maj Institute of Pharmacology using the method described by Shirota et al. [62 (link)]; both were dissolved in sterile water. All solutions were made fresh on the day of the experiment. The dose of ketamine (10 mg/kg) was based on a report by Popik et al. [63 (link)], while doses of psilocybin (2 and 10 mg/kg) were based on work by Jefsen et al. [61 (link)]. Psilocybin was given subcutaneously (sc) while ketamine was given intraperitoneally (ip) in the volume of 2 mL/kg. The control group was treated with 0.9% NaCl solution. Ketamine, xylasine hydrochlorides and sodium pentobarbital used for anesthetizing the animals came from Biowet Puławy (Puławy, Poland). All necessary chemicals of the highest purity used for analysis by high-performance liquid chromatography (HPLC) were obtained from Merck (Warsaw, Poland). O-phthalaldehyde (OPA) from Sigma-Aldrich (Poznań, Poland) was used for the derivatization of glutamate and GABA to electroactive compounds. The reagents used in immunohistochemistry were purchased from Sigma-Aldrich (Poznań, Poland), Vector Laboratories (Burlingame, CA, USA), and Proteintech (Manchester, UK).
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