Cloning and Production of Viral Vectors

ChAdOx1 and MVA expressing each antigen were cloned using GeneArt Technology (ThermoFisher Scientific, UK). Four mammalian codon-optimised antigens (fasD1, fasD2, glfT2, iniB) and flanked by a Kozak consensus sequence, a tPA leader sequence, a GS linker at the 5′-end and a PK tag, Histidine tag and STOP codon at the 3′ end, were cloned into a GeneArt entry vector and then recombined into ChAdOx1 or MVA destination plasmids as previously described.^{63 (link),64 (link)} ChAdOx1 and MVA expressing Ag85A and PPE15 were produced as previously described.^{24 (link),25} Construct sequences are available upon request.