GABA Modulation of Melanoma Protein Expression

B16F10 melanoma cells (5 × 10⁴ cells/mL) were treated with the indicated concentrations of GABA (0–20 mM) in the presence or absence of 500 ng/mL α-MSH for 72 h. Total protein was extracted using a PRO-PREP Protein Extraction Solution Kit (iNtRON Biotechnology), and total proteins were quantified using a Bio-Rad Protein Assay Kit (Bio-Rad). Western blotting was performed to detect the expression of MITF, tyrosinase, and pCREB according to our previous study [44 (link)]. β-Actin was used as an internal control.

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Molagoda I.M., Kavinda M.H., Ryu H.W., Choi Y.H., Jeong J.W., Kang S, & Kim G.Y. (2021). Gamma-Aminobutyric Acid (GABA) Inhibits α-Melanocyte-Stimulating Hormone-Induced Melanogenesis through GABAA and GABAB Receptors. International Journal of Molecular Sciences, 22(15), 8257.

Publication 2021

PRO-PREP Protein Extraction Solution Kit Bio-Rad Protein Assay Kit

Actin Assay Cells Gaba Intron Melanoma Mitf Pro protein Proteins Tyrosinase α msh

Corresponding Organization : Institute for Basic Science

Other organizations : Korea Research Institute of Bioscience and Biotechnology, Dong-Eui University, National Institute of Biological Resources, Korea Health Industry Development Institute

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Variable analysis

independent variables

GABA concentrations (0–20 mM)

dependent variables

Expression of MITF
Expression of tyrosinase
Expression of pCREB

control variables

Cell line: B16F10 melanoma cells (5 × 10^4 cells/mL)
Treatment duration: 72 h
Presence or absence of 500 ng/mL α-MSH

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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