Biomarker Measurement in Blood Samples

Blood samples were obtained, processed, refrigerated, and stored at −70 °C until analysis at the central laboratory institution (Seoul Clinical Laboratories, Yongin-si, Gyeonggi-do, Korea). The analytical methods have been published previously [20 (link),21 (link)]. Briefly, myostatin, IL-6, and TNFα levels were measured by enzyme-linked immunosorbent assay (ELISA) using DGDF80 (GDF-8/Myostatin Quantikine ELISA Kit, R&D Systems), HS600C (Human IL-6 Quantikine HS ELISA Kit), and HSTA00D (Human TNF-α Quantikine HS ELISA). Intact parathyroid hormone and 25(OH)D levels were measured by electrochemiluminescence and chemiluminescence immunoassays using a Cobas E801 analyzer (Roche Diagnostics GmbH, Mannheim, Germany). Serum total IS levels were measured using a high-performance liquid chromatography-fluorescence detector (HPLC-FLD, Agilent 1100 series; Agilent Technologies, Santa Clara, CA, USA). Serum hemoglobin, total protein, albumin, blood urea nitrogen, creatinine, CRP, and glycated hemoglobin levels were analyzed at each center.

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Lee S.M., Han M.Y., Kim S.H., Cha R.H., Kang S.H., Kim J.C, & An W.S. (2022). Indoxyl Sulfate Might Play a Role in Sarcopenia, While Myostatin Is an Indicator of Muscle Mass in Patients with Chronic Kidney Disease: Analysis from the RECOVERY Study. Toxins, 14(10), 660.

Publication 2022

GDF-8/Myostatin Quantikine ELISA Kit Human IL-6 Quantikine HS ELISA Kit Agilent 1100 series

Albumin Blood Blood urea nitrogen Chemiluminescence immunoassays Clinical laboratories Creatinine Diagnostics Enzyme linked immunosorbent assay Fluorescence Glycated hemoglobin Hemoglobin Hplc Human Myostatin Parathyroid hormone Protein Serum Tnf α

Corresponding Organization : Dong-A University

Other organizations : Hallym University, Hangang Sacred Heart Hospital, Chung-Ang University Hospital, National Medical Center, Yeungnam University, CHA University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Myostatin levels
IL-6 levels
TNFα levels
Intact parathyroid hormone levels
25(OH)D levels
Serum total IS levels
Serum hemoglobin levels
Serum total protein levels
Serum albumin levels
Serum blood urea nitrogen levels
Serum creatinine levels
Serum CRP levels
Serum glycated hemoglobin levels

control variables

None explicitly mentioned

controls

None specified

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