Fabrication of NO-Releasing PA Nanomatrices

As documented in previous work, a mixture of PA-YIGSR and PA-KKKKK at a 9:1 molar ratio was reacted with NO gas to generate PA-YK-NO.11 (link) In order to make NO-releasing PA nanomatrices, 120 μL of a 0.1% wt stock PA-YK-NO solution (pH 7.4) was prepared and dispensed into 48-well tissue culture plates (BD Biosciences, San Jose, CA, USA) with serial dilutions to make different PA-YK-NOs, ie, 130 μmol PA-YK-NO, 65 μmol PA-YK-NO, 32.5 μmol PA-YK-NO, and 16.25 μmol PA-YK-NO.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Lim D.J., Andukuri A., Vines J.B., Rahman S.M., Hwang P.T., Kim J., Shalev A., Corbett J.A, & Jun H.W. (2014). Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix. International Journal of Nanomedicine, 9(Suppl 1), 13-21.

Publication 2014

48-well tissue culture plates

Dilutions Molar Pa 11 Tissue Yigsr

Top 5 similar protocols

Variable analysis

independent variables

Molar ratio of PA-YIGSR and PA-KKKKK (9:1)
Concentration of PA-YK-NO solution (130 μmol, 65 μmol, 32.5 μmol, 16.25 μmol)

dependent variables

Formation of PA-YK-NO

control variables

PH of PA-YK-NO solution (7.4)
Tissue culture plates used (48-well, BD Biosciences, San Jose, CA, USA)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!