Mitochondrial Membrane Potential Assessment

Mitochondrial membrane potential (MMP) was measured by Rhodamine 123 (Rh123) staining (Beyotime, Nantong, China). Rh123 can selectively enter the mitochondria at full membrane potential and remain in the mitochondria [13 (link),14 (link)]. After indicated treatment, cells were incubated with 5 µg/mL Rh123 at 37 °C for 30 min in the dark. The samples were immediately analyzed at excitation wavelength of 507 nm and emission wavelength of 529 nm using a fluorescence microscope.

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Ji J., Wang K., Meng X., Zhong H., Li X., Zhao H., Xie G., Xie Y., Wang X, & Zhu X. (2022). Elaiophylin Inhibits Tumorigenesis of Human Lung Adenocarcinoma by Inhibiting Mitophagy via Suppression of SIRT1/Nrf2 Signaling. Cancers, 14(23), 5812.

Publication 2022

Rhodamine 123 (Rh123)

Cells Fluorescence microscope Mitochondria Mitochondria membrane potential Rhodamine 123

Corresponding Organization : Nantong University

Other organizations : Guangxi Medical University, Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Indicated treatment

dependent variables

Mitochondrial membrane potential (MMP) measured by Rhodamine 123 (Rh123) staining

control variables

Incubation of cells with 5 µg/mL Rh123 at 37 °C for 30 min in the dark
Excitation wavelength of 507 nm and emission wavelength of 529 nm for fluorescence microscope analysis

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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