Histopathological Evaluation of Brain Tissues

Brain tissues from mouse and porcine models [25 (link)] were fixed in 4% (w/v) polyoxymethylene solution for 48 h prior to histopathological examination. The brains were fixed by perfusion, embedded in paraffin, and stained with HE, acid vanadium fuchsin, cresyl violet, and GFAP [26 (link)]. Immunohistochemical analysis was performed with Histostain™-SP Kits (ZSbio, Beijing, China, SPN-9001) according to the manufacturer’s instructions. The primary antibodies used in this assay were rabbit anti-RPSA and -HSPD1 (1:20 dilution, Proteintech), and along rabbit anti-cleaved caspase-3, -phosphorylated p38, -phosphorylated ERK, and -phosphorylated eIF4E. (1:20 dilution, Affinity).

Free full text: Click here

Liu H., Lei S., Jia L., Xia X., Sun Y., Jiang H., Zhu R., Li S., Qu G., Gu J., Sun C., Feng X., Han W., Langford P.R, & Lei L. (2021). Streptococcus suis serotype 2 enolase interaction with host brain microvascular endothelial cells and RPSA-induced apoptosis lead to loss of BBB integrity. Veterinary Research, 52, 30.

Publication 2021

Histostain™-SP Kits -phosphorylated p38

Acid fuchsin Antibodies Assay Brains Caspase 3 Cresyl violet Dilution Eif4e Embedded paraffin Gfap Mouse Perfusion Porcine Rabbit Rpsa Tissues Vanadium

Corresponding Organization : Yangtze University

Other organizations : Jilin University, Binzhou Medical University, Imperial College London

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Tissue type (mouse vs. porcine)

dependent variables

Histopathological changes
Expression of RPSA, HSPD1, cleaved caspase-3, phosphorylated p38, phosphorylated ERK, and phosphorylated eIF4E

control variables

Fixation method (4% polyoxymethylene solution for 48 h)
Embedding (paraffin)
Staining methods (HE, acid vanadium fuchsin, cresyl violet, GFAP)
Immunohistochemical analysis (Histostain™-SP Kits, primary antibodies)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!