Quantification of Global DNA Methylation

Total genomic DNA from blood samples of the animals were isolated and analyzed for 5-methylcytidine, as described previously by our group, using the Quick-gDNA^TM MiniPrep kit from Zymo research (Irvine, CA, United States). After quantification, an equal amount of genomic DNA was used to estimate global levels of 5-methylcytosine using 5-mC DNA ELISA kit from Zymo research (Irvine, CA, United States) by following the manufacturer’s instructions as described earlier by our group (Narayanan et al., 2014 (link); Veeranki et al., 2015 (link)).

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Singh M., Hardin S.J., George A.K., Eyob W., Stanisic D., Pushpakumar S, & Tyagi S.C. (2021). Epigenetics, 1-Carbon Metabolism, and Homocysteine During Dysbiosis. Frontiers in Physiology, 11, 617953.

Publication 2020

Quick-gDNATM MiniPrep kit 5-mC DNA ELISA kit

Animals Blood Elisa Genomic Mc dna

Corresponding Organization : University of Louisville

Other organizations : Case Western Reserve University, University of Kragujevac

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Variable analysis

independent variables

Genomic DNA isolated from blood samples of animals

dependent variables

Global levels of 5-methylcytosine

control variables

Equal amount of genomic DNA used for 5-mC DNA ELISA kit

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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