ALDH, ALDH+, and CD44+/CD24 cells were first treated with vehicle control (DMSO) or treatment (ASR490, DAPT, MG132, or CQ) for prescribed doses and time points. Cell viability assays: Alamar blue (Life Technologies Corporation Eugene, OR) and EdU Cell Proliferation (using the EdU-Click 488 kit, cat# BCK-EdU488-1, Sigma), were then performed per the manufacturer’s instructions.
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