The following antibodies were used: mouse monoclonal antibodies against α-tubulin (Santa Cruz, sc-23948), Cdc27 (BD, 610454), GAPDH (Santa Cruz, sc-32233), cyclin B (Santa Cruz, sc-245), and Cdh1 (Abcam, ab3242); rabbit polyclonal antibodies against actin (Abcam, ab119716), Cdc20 (Abcam, ab64877), securin (SantaCruz, sc-56207), Lamin B (Abcam, ab16048), and pCdh1 [A gift from Dr. Dongmin Kang at Ewha Womans University22 (link)]; and a rabbit monoclonal antibody mixture against the phospho-Cdk substrate motif[(K/H)pSP] (Cell Signaling, #9477). Horseradish-peroxidase conjugated secondary antibodies were obtained from Santa Cruz. Anti-rabbit Alexa-594 antibody was purchased from Invitrogen (Invitrogen, A11012).
Protocol detail
Immunoblotting Antibody Panel for Cell Cycle Analysis
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Actin
Alexa 594
Anti antibody
Antibodies
Cdh1
Cyclin b
Gapdh
Horseradish peroxidase
Lamin b
Monoclonal antibody
Mouse
Rabbit
Securin
α tubulin
Corresponding Organization :
Other organizations : Ajou University
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Variable analysis
independent variables
- None explicitly mentioned
- None explicitly mentioned
- α-tubulin
- Cdc27
- GAPDH
- Cyclin B
- Actin
- Cdc20
- Securin
- Lamin B
- PCdh1
- Phospho-Cdk substrate motif [(K/H)pSP]
- None explicitly mentioned
- None explicitly mentioned
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