Comparative DNA Isolation Kit Evaluation

Five commercially available DNA isolation kits were compared: DNeasy Blood & Tissue Kit (Qiagen; DNE(Q)), GeneMATRIX Environmental DNA & RNA Purification Kit (Eurx; ENV(E)), GeneMATRIX Soil DNA Purification Kit (Eurx; SOI(E)), E.Z.N.A. Soil DNA Kit (Omega Bio‐tek; SOI(O)) and E.Z.N.A. Water DNA Kit (Omega Bio‐tek; WAT(O)). The selected methods are commonly used in metabarcoding surveys (Kaden and Krolla‐Sidenstein, 2015 (link); Braun and Szewzyk, 2016 (link); Wang et al., 2018 (link); Gołębiewski and Tretyn, 2019 (link); Jeunen et al., 2019 (link)). All isolation steps were performed according to the manufacturer's instructions. Finally, the DNA was eluted or suspended in 100 μl of nuclease‐free water (GE Healthcare).

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Gumińska N., Łukomska‐Kowalczyk M., Chaber K., Zakryś B, & Milanowski R. (2021). Evaluation of V2 18S rDNA barcode marker and assessment of sample collection and DNA extraction methods for metabarcoding of autotrophic euglenids. Environmental Microbiology, 23(6), 2992-3008.

Publication 2021

DNeasy Blood & Tissue Kit GeneMATRIX Soil DNA Purification Kit E.Z.N.A. Soil DNA Kit E.Z.N.A. Water DNA Kit nuclease‐free water

Blood Environmental dna Isolation Tissue

Corresponding Organization : University of Warsaw

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