Atovaquone Antibody Staining Protocol
Corresponding Organization : Baylor College of Medicine
Other organizations : Texas Children's Hospital, Rice University, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Center for Cancer and Blood Disorders, Seattle Children's Hospital
Variable analysis
- Atovaquone purchased from Sigma (A7986) for in vitro work
- Atovaquone purchased from Amneal Pharmaceuticals for in vivo studies
- Measured outcomes
- Fluorochrome-conjugated antibody to pS6-AF647 (Ser240/244) from Cell Signaling Technologies
- CD130-AlexaFluor 488 from Abcam
- PY-STAT3-PE and isotype control from BD Biosciences
- Positive control: Not specified
- Negative control: Not specified
Annotations
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