The concentrated hydrolysed collagen was analysed by physical–chemical methods according to the standards in force and methods such as ash content (EN ISO 4047:2008), dry matter (EN ISO 4684:2006), total nitrogen and protein (ISO 5397:1996), pH (STAS 8619/3:1990), amino nitrogen (according to ICPI method), and electrical conductivity (EN ISO 27883:1997). The viscosity of concentrated hydrolysed collagen hydrolysate was determined with a Brookfield AMETEK DV2T TC-550 Viscometer (Brookfield, Toronto, ON, Canada) at a temperature of 25 °C. The particle size and zeta potential of collagen were evaluated by using a Dynamic Light Scattering (DLS) instrument from Malvern (Zetasizer Nano-ZS, Malvern Hills, UK).
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