Western Blot Analysis of Signaling Proteins

After extraction of total and nuclear proteins, western blotting was performed with primary antibody against JNK (1:1000, Cell Signaling Technology), phospho-JNK (1:1000, Cell Signaling Technology), ERK1/2 (1:1000, Proteintech), phospho-ERK1/2 (1:1000, Cell Signaling Technology), FOXO1 (1:1000, Proteintech), BAX (1:1000, Proteintech), CASP3 (1:1000, Proteintech), histone H3 (1:5000, Proteintech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:5000, Proteintech) as described previously 16 (link).

Free full text: Click here

Huang J.C., Duan C.C., Jin S., Sheng C.B., Wang Y.S., Yue Z.P, & Guo B. (2022). HB-EGF induces mitochondrial dysfunction via estrogen hypersecretion in granulosa cells dependent on cAMP-PKA-JNK/ERK-Ca2+-FOXO1 pathway. International Journal of Biological Sciences, 18(5), 2047-2059.

Publication 2022

phospho-JNK ERK1/2 phospho-ERK1/2 FOXO1 BAX CASP3 histone H3 GAPDH

Antibody Casp3 Erk1 Gapdh Glyceraldehyde 3 phosphate dehydrogenase Histone h3 Nuclear proteins

Corresponding Organization : Jilin University

Other organizations : Changchun University, Second Affiliated Hospital of Jilin University

Top 5 similar protocols

Variable analysis

independent variables

Primary antibody against JNK (1:1000, Cell Signaling Technology)
Primary antibody against phospho-JNK (1:1000, Cell Signaling Technology)
Primary antibody against ERK1/2 (1:1000, Proteintech)
Primary antibody against phospho-ERK1/2 (1:1000, Cell Signaling Technology)
Primary antibody against FOXO1 (1:1000, Proteintech)
Primary antibody against BAX (1:1000, Proteintech)
Primary antibody against CASP3 (1:1000, Proteintech)
Primary antibody against histone H3 (1:5000, Proteintech)
Primary antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:5000, Proteintech)

dependent variables

Total and nuclear protein levels

control variables

Western blotting protocol as described previously 16

controls

No positive or negative controls are explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!