The siGenome siRNAs (Dharmacon, Lafayette, CO) were used to target individual genes for inhibiting their expression as previously described (7 (link)). Cells were transfected with a negative control siRNA or a specific siRNA using DharmaFECT 4 reagent (Dharmacon, Lafayette, CO). A mixture of siRNA (25 nM final concentration) and DharmaFECT 4 reagent was incubated for 20 minutes at room temperate before being applied to the cells.
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Gene Expression Silencing using siRNAs
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Corresponding Organization : The University of Texas MD Anderson Cancer Center
Other organizations : Soochow University, First Affiliated Hospital of Soochow University, Arrien Pharmaceuticals (United States), University of Oxford, National Institute of Biomedical Innovation, Health and Nutrition
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Variable analysis
independent variables
- Specific siRNA targeting individual genes
- Expression of genes targeted by the siRNAs
- Negative control siRNA
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