Histological scoring of brain damage

Selected brain sections were fixed in 10% buffered formalin (Kulich, Hradec Kralove, Czech Republic), processed through conventional histological techniques, and stained with hematoxylin and eosin (both Merck, Kenilworth, NJ, USA). The histological changes were scored using a BX-51 microscope (Olympus, Tokyo, Japan) and following semi-quantitative criteria published previously [20 (link)]:

(0)

no pathology,

(1)

mild damage: 1–2 neurons with nucleus margination, chromatolysis and/or vacuolar degeneration,

(2)

moderate damage: ≥ 3 neurons with changes described in (1) and/or ≥ 1 shrunken eosinophilic neurons in 1 nucleus/subregion,

(3)

severe damage: focal damage or multiple changes described in (2) present in ≥2 nuclei/subregions and/or hemorrhage

(4)

very severe damage: diffuse damage of the region and/or multiple hemorrhages

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Caisberger F., Pejchal J., Misik J., Kassa J., Valis M, & Kuca K. (2018). The benefit of combinations of oximes for the ability of antidotal treatment to counteract sarin-induced brain damage in rats. BMC Pharmacology & Toxicology, 19, 35.

Publication 2018

hematoxylin and eosin BX-51 microscope

Brain Eosin Eosinophilic Formalin Hemorrhages Histological techniques Margination nucleus Microscope Neurons Nuclei Vacuolar

Corresponding Organization : University of Hradec Králové

Other organizations : University Hospital Hradec Králové, University of Defence

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Histological changes in brain sections

control variables

Brain sections were fixed in 10% buffered formalin
Brain sections were processed through conventional histological techniques
Brain sections were stained with hematoxylin and eosin

controls

Positive control: Not mentioned
Negative control: Not mentioned

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